7N5S

ZBTB7A Zinc Finger Domain Bound to -200 Site of Fetal Globin Promoter (Oligo 6)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00000
Spacegroup name	P 1 2 1
Unit cell lengths	46.938, 36.963, 74.257
Unit cell angles	90.00, 100.01, 90.00

Refinement procedure

Resolution	36.320 - 2.860
R-factor	0.2283
Rwork	0.226
R-free	0.27220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7n5u
RMSD bond length	0.003
RMSD bond angle	0.427
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.320	2.950
High resolution limit [Å]	2.850	2.850
Rmerge	0.097	1.670
Rpim	0.030	0.701
Number of reflections	10160	628
<I/σ(I)>	18.8	1.4
Completeness [%]	92.6	66
Redundancy	10.3	4.1
CC(1/2)		0.610

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	289	0.2 M trimethylamine N-oxide dihydrate, 0.1 M Tris, pH 8.5, 20% w/v PEG2000 MME