7N5N
PCNA from Thermococcus gammatolerans: crystal III, collection 15, 2.20 A, 28.7 MGy
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-04-25 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 3 |
Unit cell lengths | 93.056, 93.056, 63.976 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 34.095 - 2.200 |
R-factor | 0.1788 |
Rwork | 0.176 |
R-free | 0.22570 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5a6d |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.095 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.131 | 0.986 |
Number of reflections | 31432 | 3150 |
<I/σ(I)> | 19.02 | 2.37 |
Completeness [%] | 99.6 | 99.75 |
Redundancy | 5.1 | 5.1 |
CC(1/2) | 0.999 | 0.748 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 5.2 | 291 | 100 mM sodium citrate pH 5.2, 3.0 M ammonium sulfate, 7.5% MPD |