7N5M

PCNA from Thermococcus gammatolerans: crystal III, collection 1, 2.00 A, 1.91 MGy

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 3
Unit cell lengths	92.616, 92.616, 63.730
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.943 - 2.000
R-factor	0.1905
Rwork	0.188
R-free	0.23890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5a6d
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.943	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.124	0.909
Number of reflections	41186	4088
<I/σ(I)>	20.57	2.52
Completeness [%]	99.5	98.91
Redundancy	5.2	4.8
CC(1/2)	0.999	0.845

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	5.2	291	100 mM sodium citrate pH 5.2, 3.0 M ammonium sulfate, 7.5% MPD