7N5K
PCNA from Thermococcus gammatolerans: crystal II, collection 1, 1.98 A, 3.84 MGy
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-04-25 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 63 |
Unit cell lengths | 186.920, 186.920, 64.031 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 32.125 - 1.980 |
R-factor | 0.1917 |
Rwork | 0.190 |
R-free | 0.22760 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5a6d |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.125 | 2.050 |
High resolution limit [Å] | 1.980 | 1.980 |
Rmerge | 0.224 | 1.004 |
Number of reflections | 89151 | 8861 |
<I/σ(I)> | 24.34 | 2.35 |
Completeness [%] | 99.9 | 99.86 |
Redundancy | 10.2 | 9.8 |
CC(1/2) | 0.999 | 0.828 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 5.2 | 291 | 100 mM sodium citrate pH 5.2, 3.2 M ammonium sulfate, 7.5% MPD |