7N5K

PCNA from Thermococcus gammatolerans: crystal II, collection 1, 1.98 A, 3.84 MGy

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 63
Unit cell lengths	186.920, 186.920, 64.031
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.125 - 1.980
R-factor	0.1917
Rwork	0.190
R-free	0.22760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5a6d
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.125	2.050
High resolution limit [Å]	1.980	1.980
Rmerge	0.224	1.004
Number of reflections	89151	8861
<I/σ(I)>	24.34	2.35
Completeness [%]	99.9	99.86
Redundancy	10.2	9.8
CC(1/2)	0.999	0.828

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	5.2	291	100 mM sodium citrate pH 5.2, 3.2 M ammonium sulfate, 7.5% MPD