7N5J

PCNA from Thermococcus gammatolerans: crystal I, collection 5, 2.82 A, 89.1 MGy

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 63
Unit cell lengths	187.870, 187.870, 64.314
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.326 - 2.820
R-factor	0.1736
Rwork	0.170
R-free	0.23300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5a6d
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.330	2.920
High resolution limit [Å]	2.820	2.820
Rmerge	0.152	1.205
Number of reflections	31591	3147
<I/σ(I)>	18.68	2.43
Completeness [%]	99.8	99.9
Redundancy	10.3	10.5
CC(1/2)	0.979	0.797

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	5.2	291	100 mM sodium citrate pH 5.2, 3.2 M ammonium sulfate, 7.5% MPD