7N57
Crystal structure of R20A human Galectin-7 mutant in presence of lactose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-06-09 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97952 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 30.220, 76.190, 111.190 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.910 - 1.830 |
R-factor | 0.173 |
Rwork | 0.171 |
R-free | 0.20820 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4gal |
RMSD bond length | 0.011 |
RMSD bond angle | 1.206 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (2.7.12) |
Refinement software | PHENIX (1.19.2-4158) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 44.910 | 44.910 | 1.500 |
High resolution limit [Å] | 1.460 | 6.530 | 1.460 |
Rmerge | 0.186 | 0.119 | 1.755 |
Rmeas | 0.202 | 0.133 | 1.890 |
Total number of observations | 322349 | ||
Number of reflections | 45632 | 599 | 3336 |
<I/σ(I)> | 5.12 | 10.8 | 1.01 |
Completeness [%] | 100.0 | 99.7 | 100 |
Redundancy | 7.064 | 5.953 | 7.204 |
CC(1/2) | 0.986 | 0.985 | 0.346 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.2M Sodium chloride, 0.1M Tris pH 8.0, 20% w/v PEG 6000 |