7N50
Structure of a bacterial gasdermin from Bradyrhizobium tropiciagri
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2020-11-04 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97918 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 40.770, 146.180, 34.980 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.270 - 1.500 |
R-factor | 0.1909 |
Rwork | 0.188 |
R-free | 0.22020 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.052 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX (1.19.1_4122) |
Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.770 | 1.530 |
High resolution limit [Å] | 1.500 | 1.500 |
Rpim | 0.038 | 0.787 |
Number of reflections | 33993 | 1639 |
<I/σ(I)> | 12.3 | 2 |
Completeness [%] | 98.7 | |
Redundancy | 7.4 | |
CC(1/2) | 0.998 | 0.216 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.4 | 291 | 100 mM sodium acetate (pH 4.4) and 23% PEG-3350 |