7N4P
Crystal Structure of Lizard Cadherin-23 EC1-2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-12-18 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97920 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 43.386, 43.386, 296.227 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.671 - 2.097 |
Rwork | 0.211 |
R-free | 0.26650 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2whv |
RMSD bond length | 0.014 |
RMSD bond angle | 1.857 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.140 |
High resolution limit [Å] | 2.097 | 2.097 |
Rmeas | 0.106 | 0.589 |
Number of reflections | 17931 | 736 |
<I/σ(I)> | 16.65 | |
Completeness [%] | 98.3 | 86 |
Redundancy | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 0.1 M MES pH 6.5 1.1 M CaCl2 |