7N4D

Translation initiation factor eif-5a family protein from Naegleria fowleri ATCC 30863

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2021-03-25
Detector	RAYONIX MX-300
Wavelength(s)	0.97872
Spacegroup name	P 1 21 1
Unit cell lengths	57.570, 58.940, 102.340
Unit cell angles	90.00, 93.57, 90.00

Refinement procedure

Resolution	31.360 - 2.450
R-factor	0.2387
Rwork	0.234
R-free	0.29390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5dlq as per Morda
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MoRDa
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	31.360	31.360	2.510
High resolution limit [Å]	2.450	10.960	2.450
Rmerge	0.067	0.023	0.527
Rmeas	0.084	0.029	0.657
Total number of observations	65938
Number of reflections	24974	266	1858
<I/σ(I)>	11.39	33.14	2.11
Completeness [%]	98.2	84.2	99.5
Redundancy	2.64	2.44	2.645
CC(1/2)	0.997	0.998	0.792

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		287	Rigaku JCSG+ screen, D12:0.04M KPO4, 16% w/v PEG 8 000, 20% glycerol: NafoA.20237.a.D11.PD38375 NafoA.01541.a.B2.PW38668 (4:1) at 13.91 mg/ml + 4mM GC7,deoxyhypusine synthase inhibitor + 4mM NAD: cryo: direct: tray 320284d12, puck pvq5-1