7N3S
Human bisphosphoglycerate mutase complex with 2-phosphoglycolate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-03-22 |
Detector | DECTRIS EIGER R 4M |
Wavelength(s) | 1.54056 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 52.643, 70.808, 158.933 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.999 - 2.480 |
R-factor | 0.2135 |
Rwork | 0.210 |
R-free | 0.28430 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2h4z |
Data reduction software | CrysalisPro (41_64_69a) |
Data scaling software | Aimless (0.5.32) |
Phasing software | PHENIX (1.9-1692) |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.000 | 2.580 |
High resolution limit [Å] | 2.480 | 2.480 |
Rmerge | 0.111 | 0.710 |
Rmeas | 0.120 | 0.772 |
Rpim | 0.047 | 0.300 |
Total number of observations | 140609 | |
Number of reflections | 21810 | 2412 |
<I/σ(I)> | 9.7 | |
Completeness [%] | 99.9 | 100 |
Redundancy | 6.4 | 6.5 |
CC(1/2) | 0.997 | 0.881 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 0.1 M BIS TRIS propane, 18% polyethylene glycol 8000, 10% polyethylene glycol 200 |