7N39
Crystal structure of 7-site deamidated variant of human gamma(S)-crystallin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P14 (MX2) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-06-09 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9763 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 49.146, 76.629, 94.655 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.270 - 1.560 |
R-factor | 0.1944 |
Rwork | 0.193 |
R-free | 0.21270 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6fd8 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.813 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.270 | 1.620 |
High resolution limit [Å] | 1.560 | 1.560 |
Number of reflections | 51461 | 5051 |
<I/σ(I)> | 27.17 | |
Completeness [%] | 99.6 | |
Redundancy | 13.3 | |
CC(1/2) | 0.999 | 0.927 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M sodium acetate pH 5.0, 0.1 M ammonium sulfate, 28% PEG 3350 |