7N35

Structure of Yersinia aleksiciae Cap15 cyclic dinucleotide receptor, crystal form 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	80
Detector technology	PIXEL
Collection date	2020-12-09
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97918
Spacegroup name	P 61
Unit cell lengths	50.866, 50.866, 155.018
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.050 - 2.600
R-factor	0.299
Rwork	0.297
R-free	0.31300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7n34
RMSD bond length	0.002
RMSD bond angle	0.491
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.050	2.720
High resolution limit [Å]	2.600	2.600
Rpim	0.018	0.674
Number of reflections	7040	853
<I/σ(I)>	19.3	1.3
Completeness [%]	100.0
Redundancy	15.9
CC(1/2)	1.000	0.512

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.3	291	40% PEG-200, 0.1 M N-(2-Acetamido)iminodiacetic acid pH 5.3