7N34

Structure of Yersinia aleksiciae Cap15 cyclic dinucleotide receptor, crystal form 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	80
Detector technology	PIXEL
Collection date	2020-11-04
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97918
Spacegroup name	C 1 2 1
Unit cell lengths	93.292, 31.039, 50.019
Unit cell angles	90.00, 100.22, 90.00

Refinement procedure

Resolution	37.010 - 1.900
R-factor	0.2184
Rwork	0.214
R-free	0.25430
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	0.752
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.010	1.940
High resolution limit [Å]	1.900	1.900
Rpim	0.029	0.643
Number of reflections	11344	719
<I/σ(I)>	13.3	1.3
Completeness [%]	99.8
Redundancy	6.9
CC(1/2)	0.999	0.562

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.8	291	40% PEG-200, 0.1 M N-(2-Acetamido)iminodiacetic acid pH 5.8