7N2R

AS4.3-PRPF3-HLA*B27

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2019-12-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97946
Spacegroup name	P 21 21 21
Unit cell lengths	54.680, 94.423, 178.522
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.260 - 2.280
R-factor	0.2497
Rwork	0.248
R-free	0.28810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1jge 5ksb
RMSD bond length	0.003
RMSD bond angle	0.537
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19_4080)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.810	2.360
High resolution limit [Å]	2.280	2.280
Number of reflections	42767	4065
<I/σ(I)>	7.6
Completeness [%]	99.7
Redundancy	13.1
CC(1/2)	0.996	0.339

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	25% PEG3350 with 0.2M Ammonium sulfate and 0.1M BIS-TRIS pH 6.5