7N2P

AS4.3-RNASEH2b-HLA*B27

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 2.0.1
Synchrotron site	ALS
Beamline	2.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-01
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.046
Spacegroup name	P 21 21 21
Unit cell lengths	54.925, 93.992, 177.774
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.000 - 2.500
R-factor	0.2355
Rwork	0.230
R-free	0.28840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ksb 1jge
RMSD bond length	0.002
RMSD bond angle	0.454
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.000	2.589
High resolution limit [Å]	2.500	2.500
Rmeas	0.278	2.427
Rpim	0.130	1.123
Number of reflections	32459	3146
<I/σ(I)>	5.88	0.79
Completeness [%]	99.1	97.94
Redundancy	4.4	4.4
CC(1/2)	0.985	0.318

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	20% PEG3350 with 0.2M Magnesium formate dihydrate