7N2L

MicroED structure of a mutant mammalian prion segment

Experimental procedure

Spacegroup name	P 1
Unit cell lengths	4.870, 10.060, 30.660
Unit cell angles	94.85, 90.26, 99.99

Refinement procedure

Resolution	7.980 - 0.900
R-factor	0.1967
Rwork	0.197
R-free	0.19800
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	7.980	7.980	0.930
High resolution limit [Å]	0.900	1.930	0.900
Rmerge	0.197	0.151	0.475
Rmeas	0.214	0.163	0.529
Total number of observations	18502
Number of reflections	3437	350	330
<I/σ(I)>	5.21	8.61	2.56
Completeness [%]	81.0	79	82.3
Redundancy	5.383	5.834	4.858
CC(1/2)	0.988	0.986	0.783

Crystallization Conditions

crystal ID	method	pH	temperature	details
1