7N2H
X-Ray structure of a sequence variant of a repeat segment of the yeast prion New1p
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-12-08 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979 |
Spacegroup name | P 31 |
Unit cell lengths | 27.200, 27.200, 4.830 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 13.600 - 1.102 |
R-factor | 0.1408 |
Rwork | 0.138 |
R-free | 0.16060 |
Structure solution method | AB INITIO PHASING |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHELXD |
Refinement software | PHENIX (1.16-3549-000) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 13.600 | 13.600 | 1.100 |
High resolution limit [Å] | 1.050 | 2.100 | 1.050 |
Rmerge | 0.161 | 0.114 | 0.359 |
Rmeas | 0.197 | 0.139 | 0.447 |
Total number of observations | 4635 | ||
Number of reflections | 1793 | 238 | 154 |
<I/σ(I)> | 4.13 | 6.43 | 1.97 |
Completeness [%] | 90.2 | 94.8 | 61.1 |
Redundancy | 2.585 | 2.639 | 2.039 |
CC(1/2) | 0.974 | 0.971 | 0.891 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298 | 20% MPD, 0.1 M sodium acetate, pH 4.5 |