7N1J
Crystal structure of FGFR4 domain 3 in complex with a de novo-designed mini-binder
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-03-10 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 63 |
Unit cell lengths | 107.531, 107.531, 69.047 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.560 - 2.990 |
R-factor | 0.2099 |
Rwork | 0.209 |
R-free | 0.23290 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1cvs |
RMSD bond length | 0.003 |
RMSD bond angle | 0.481 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.170 |
High resolution limit [Å] | 2.990 | 2.990 |
Number of reflections | 9194 | 1452 |
<I/σ(I)> | 19.1 | |
Completeness [%] | 98.7 | |
Redundancy | 4.7 | |
CC(1/2) | 0.998 | 0.952 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277.15 | 0.2 M sodium chloride, 0.1 M MES (pH 6.0), 20% PEG 3,350 |