7N04

Crystal structure of the apo F240 antibody fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-12-04
Detector	RAYONIX MX-300
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	46.996, 74.382, 145.041
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.708 - 1.700
R-factor	0.180877855087
Rwork	0.179
R-free	0.22188
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dfb
RMSD bond length	0.010
RMSD bond angle	1.064
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.710	1.760
High resolution limit [Å]	1.700	1.700
Rmerge	0.023	0.056
Number of reflections	54928	4303
<I/σ(I)>	11.6
Completeness [%]	96.6	76.7
Redundancy	2
CC(1/2)	1.000	0.530

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.1 M HEPES-NaOH, pH 7.5, and 20% (w/v) PEG 8000