7MZO
Crystal structure of the UcaD lectin-binding domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-03-30 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9537 |
Spacegroup name | I 4 |
Unit cell lengths | 77.390, 77.390, 70.910 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.700 - 1.620 |
R-factor | 0.1699 |
Rwork | 0.169 |
R-free | 0.19220 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | UclD |
RMSD bond length | 0.009 |
RMSD bond angle | 0.957 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 54.720 | 1.650 |
High resolution limit [Å] | 1.620 | 1.620 |
Rmerge | 0.110 | 1.443 |
Rmeas | 0.119 | 1.559 |
Rpim | 0.045 | 0.584 |
Number of reflections | 26588 | 1305 |
<I/σ(I)> | 11.6 | 1.4 |
Completeness [%] | 99.9 | |
Redundancy | 7 | |
CC(1/2) | 0.999 | 0.634 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M sodium citrate buffer pH 4.5-5.5; 2-3 M NaCl |