7MYS

Crystal structure of a tRNA (guanine-N1)-methyltransferase from Acinetobacter baumannii AB5075-UW bound to S-adenosyl homocysteine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2021-02-25
Detector	RAYONIX MX-300
Wavelength(s)	0.9787
Spacegroup name	P 41
Unit cell lengths	65.960, 65.960, 132.590
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.640 - 2.400
R-factor	0.185
Rwork	0.181
R-free	0.22520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ual
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (dev-4224-000)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.640	46.640	2.460
High resolution limit [Å]	2.400	10.730	2.400
Rmerge	0.040	0.023	0.524
Rmeas	0.043	0.026	0.571
Total number of observations	140392
Number of reflections	22187	259	1654
<I/σ(I)>	25.84	57.93	3.69
Completeness [%]	100.0	97	100
Redundancy	6.328	5.521	6.387
CC(1/2)	1.000	0.999	0.910

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	287	AcbaC.00054.a.B1.PW38905 at 26 mg/mL with 6 mM GMP and 6 mM SAH against Morpheus condition F2 with 10% PEG 8000, 20% ethylene glycol, 0.1 M MES/imidazole pH 6.5, 0.02 M each glucose, mannose, galactose, fucose, xylose, acetyl-glucosamine, direct cryo, unique puck ID okj1-10, crystal tracking ID 319640f2