7MYG
M. tb Ag85C modified by THL-10d
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2021-02-16 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9787 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 59.458, 70.606, 68.590 |
Unit cell angles | 90.00, 89.97, 90.00 |
Refinement procedure
Resolution | 59.460 - 2.510 |
R-factor | 0.1623 |
Rwork | 0.155 |
R-free | 0.22240 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1dqz |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 59.460 | 2.600 |
High resolution limit [Å] | 2.510 | 2.510 |
Number of reflections | 19458 | 1920 |
<I/σ(I)> | 15.35 | |
Completeness [%] | 98.0 | 94.32 |
Redundancy | 5.5 | 5.2 |
CC(1/2) | 0.822 | 0.222 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298.15 | 8% v/v Tacsimate (pH 7.0) and 20 % w/v Polyethylene glycol 3,350 |