7MY4
Crystal Structure of the SPA17 Docking and Dimerization Domain from Danio rerio
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2020-02-13 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | P 32 |
Unit cell lengths | 60.957, 60.957, 89.021 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 34.030 - 1.720 |
R-factor | 0.1551 |
Rwork | 0.154 |
R-free | 0.16450 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2izx |
RMSD bond length | 0.008 |
RMSD bond angle | 0.923 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.030 | 1.770 |
High resolution limit [Å] | 1.720 | 1.720 |
Rmerge | 0.269 | |
Rmeas | 0.098 | |
Rpim | 0.035 | |
Number of reflections | 38456 | 2519 |
<I/σ(I)> | 1.02 | |
Completeness [%] | 98.5 | 95 |
Redundancy | 7.6 | 5.6 |
CC(1/2) | 0.993 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 0.1 M HEPES pH 7.5, 0.5 M magnesium formate dihydrate |