7MXW
Crystal structure of human exonuclease 1 Exo1 (WT) in complex with 5' flap DNA (uf1)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-12-16 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 71.955, 71.955, 179.874 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.298 - 2.836 |
R-factor | 0.223 |
Rwork | 0.219 |
R-free | 0.26530 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5v05 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.899 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.900 |
High resolution limit [Å] | 2.836 | 2.850 |
Rmerge | 0.110 | 0.619 |
Number of reflections | 10413 | 261 |
<I/σ(I)> | 28.38 | |
Completeness [%] | 87.5 | |
Redundancy | 16.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 290 | 100 mM sodium acetate, pH 7.0; 10 mM KCl; 2mM CaCl2; 20 mM MgCl2; 2-4% PEG 4000 |