7MXK
Crystal structure of the S/T protein kinase PknG from Corynebacterium glutamicum (residues 130-433) in complex with AMP-PNP, isoform 2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-02-22 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.976251 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 37.807, 54.594, 146.492 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.770 - 1.990 |
R-factor | 0.2102 |
Rwork | 0.209 |
R-free | 0.23700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7mxb |
RMSD bond length | 0.003 |
RMSD bond angle | 0.613 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.830 | 2.040 |
High resolution limit [Å] | 1.990 | 1.990 |
Rmerge | 0.067 | 0.654 |
Rmeas | 0.073 | 0.725 |
Rpim | 0.029 | 0.306 |
Number of reflections | 21235 | 1207 |
<I/σ(I)> | 17.1 | |
Completeness [%] | 98.4 | 84.9 |
Redundancy | 6 | 5.2 |
CC(1/2) | 0.999 | 0.751 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 291 | 100 mM Tris-HCl, 27-30% w/v PEG 4 k, 200 mM MgCl2, pH 8.8 |