7MXJ

Crystal structure of the S/T protein kinase PknG from Corynebacterium glutamicum (residues 130-433) in complex with AMP-PNP, isoform 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-02-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.976251
Spacegroup name	P 21 21 21
Unit cell lengths	37.616, 55.936, 123.945
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.520 - 1.920
R-factor	0.2001
Rwork	0.199
R-free	0.22460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7mxb
RMSD bond length	0.003
RMSD bond angle	0.686
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.520	1.970
High resolution limit [Å]	1.920	1.920
Rmerge	0.070	0.746
Rmeas	0.077	0.823
Rpim	0.031	0.343
Number of reflections	20752	1378
<I/σ(I)>	13.6	1.9
Completeness [%]	99.9
Redundancy	5.9
CC(1/2)	0.999	0.805

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291	100 mM Tris-HCl, 27-30% w/v PEG 4 k, 200 mM MgCl2, pH 8.8