7MWW
Structure of hepatitis C virus envelope full-length glycoprotein 2 (eE2) from J6 genotype
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-08-18 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9793 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 95.840, 155.561, 129.420 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.334 - 2.710 |
| R-factor | 0.2205 |
| Rwork | 0.218 |
| R-free | 0.27020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4web |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.847 |
| Data reduction software | MOSFLM (7.2.2) |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.340 | 2.807 |
| High resolution limit [Å] | 2.710 | 2.710 |
| Number of reflections | 49955 | 2583 |
| <I/σ(I)> | 6.3 | |
| Completeness [%] | 99.0 | |
| Redundancy | 1.98 | |
| CC(1/2) | 0.980 | 0.480 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 277 | 4% v/v tacsimate pH 5.0, 14% w/v PEG3350, and 4% D-(+)-trehalose dihydrate |
