7MWL

The TAM domain of BAZ2A in complex with a 12mer mCG DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979176
Spacegroup name	P 61 2 2
Unit cell lengths	73.202, 73.202, 262.068
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.630 - 1.840
R-factor	0.2135
Rwork	0.213
R-free	0.22970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3c2i
RMSD bond length	0.013
RMSD bond angle	1.338
Data scaling software	Aimless (0.5.32)
Phasing software	Aimless
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	37.440	37.440	1.880
High resolution limit [Å]	1.840	9.020	1.840
Rmerge	0.049	0.026	0.951
Rmeas	0.050	0.027	0.976
Rpim	0.008	0.005	0.214
Total number of observations	1321725
Number of reflections	37208	424	2209
<I/σ(I)>	48.5		3.8
Completeness [%]	99.9	99	99.1
Redundancy	35.5	27.1	20
CC(1/2)	1.000	0.998	0.872

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	10% PEG 3350, 0.2M L-Proline, 0.1M Hepes pH7.5