7MWK

Crystal structure of MBD2 with DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2013-08-16
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9793
Spacegroup name	P 31
Unit cell lengths	40.521, 40.521, 202.097
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.575 - 2.453
R-factor	0.1924
Rwork	0.192
R-free	0.21650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6C2E
Data scaling software	Aimless (0.1.29)
Phasing software	PHASER
Refinement software	REFMAC (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	34.575	34.570	2.550
High resolution limit [Å]	2.450	8.830	2.450
Rmerge	0.057	0.026	1.063
Rmeas	0.062	0.029	1.167
Rpim	0.026	0.012	0.480
Total number of observations		1548	9191
Number of reflections	27350	293	1578
<I/σ(I)>	18.9	54.1	1.8
Completeness [%]	99.9	97.9	100
Redundancy	5.8	5.3	5.8
CC(1/2)	0.999	0.999	0.575

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	20% PEG3350, 0.2 M magnesium acetate