7MWH

Crystal structure of BAZ2A with DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2014-03-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.978565
Spacegroup name	P 61 2 2
Unit cell lengths	72.140, 72.140, 259.350
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.070 - 2.280
R-factor	0.2197
Rwork	0.218
R-free	0.24690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3c2i
RMSD bond length	0.009
RMSD bond angle	1.667
Data scaling software	Aimless (0.2.17)
Refinement software	REFMAC (5.8.0257)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	39.870	39.870	2.360
High resolution limit [Å]	2.280	8.830	2.280
Rmerge	0.089	0.034	1.119
Rmeas	0.089	0.035	1.119
Rpim	0.020	0.009	0.247
Number of reflections	19242	433	1821
<I/σ(I)>	23.8
Completeness [%]	100.0	98.9	100
Redundancy	20	15.5	21.3
CC(1/2)	0.999	1.000	0.891

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	20% PEG 3350, 0.2M Ammonium Chloride