7MW0
Crystal structure of Homo sapiens NUP93 solenoid (residues 174-819)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-12-18 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97946 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 74.870, 66.070, 79.180 |
Unit cell angles | 90.00, 116.28, 90.00 |
Refinement procedure
Resolution | 40.000 - 2.000 |
R-factor | 0.2262 |
Rwork | 0.225 |
R-free | 0.25440 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5hb3 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.452 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.071 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.088 | 2.537 |
Rmeas | 0.090 | 2.598 |
Rpim | 0.020 | 0.555 |
Number of reflections | 46714 | 4598 |
<I/σ(I)> | 26 | 1.54 |
Completeness [%] | 99.1 | 98.27 |
Redundancy | 20.9 | 21.5 |
CC(1/2) | 1.000 | 0.733 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 294 | 0.075 M TRIS pH 8.5, 19 % (w/v) PEG 20000 |