7MUB
KcsA Open gate E71V mutant in Potassium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 98 |
| Detector technology | PIXEL |
| Collection date | 2021-02-10 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | I 4 |
| Unit cell lengths | 139.449, 139.449, 69.219 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.000 |
| R-factor | 0.1968 |
| Rwork | 0.197 |
| R-free | 0.26000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5vk6 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.877 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 98.610 | 3.180 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 13389 | 2171 |
| <I/σ(I)> | 12.5 | |
| Completeness [%] | 99.5 | |
| Redundancy | 6.5 | |
| CC(1/2) | 0.999 | 0.357 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 50 mM Sodium acetate/Ammonium acetate, 50 mM Magnesium acetate, 25% PEG 400 |
