7MTO

Crystal Structure of SET/I2PP2A/TAF-1Beta core

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	93
Detector technology	PIXEL
Collection date	2020-07-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 21 2 21
Unit cell lengths	53.713, 55.275, 79.934
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.521 - 1.791
R-factor	0.1915
Rwork	0.189
R-free	0.23900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2e50
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.830
High resolution limit [Å]	1.791	4.880	1.791
Rmerge	0.051	0.033	0.237
Rmeas	0.059	0.038	0.279
Rpim	0.029	0.019	0.145
Number of reflections	22528	1204	1076
<I/σ(I)>	10.8
Completeness [%]	97.9	94.4	99
Redundancy	3.7	3.5	3.4
CC(1/2)		0.997	0.977

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.4	291	0.1 M Imidazole, 0.2M calcium acetate, 22% PEG 1000