7MT1
Crystal structure of Human Platelet-activating factor acetylhydrolase IB subunit beta (PAFAH1B1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-04-11 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.728, 68.572, 69.549 |
| Unit cell angles | 90.00, 101.28, 90.00 |
Refinement procedure
| Resolution | 36.030 - 1.300 |
| R-factor | 0.1775 |
| Rwork | 0.177 |
| R-free | 0.19670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vyh |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.431 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 36.040 | 36.020 | 1.320 |
| High resolution limit [Å] | 1.300 | 7.120 | 1.300 |
| Rmerge | 0.034 | 0.021 | 0.477 |
| Rmeas | 0.037 | 0.023 | 0.531 |
| Rpim | 0.015 | 0.009 | 0.228 |
| Total number of observations | 2989 | 20345 | |
| Number of reflections | 82396 | 533 | 3866 |
| <I/σ(I)> | 22.5 | 65.8 | 3.1 |
| Completeness [%] | 99.2 | 98.9 | 94.4 |
| Redundancy | 5.6 | 5.6 | 5.3 |
| CC(1/2) | 1.000 | 0.999 | 0.862 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 295 | 18% PEG8K, 0.1M Tris pH 8.5 |
