7MSU

Crystal structure of an archaeal CNNM, MtCorB, CBS-pair domain in complex with Mg2+-ATP

Replaces:  7LJ6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.00003
Spacegroup name	P 41 2 2
Unit cell lengths	52.100, 52.100, 112.284
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.190 - 2.070
R-factor	0.2083
Rwork	0.206
R-free	0.25170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5yz2
RMSD bond length	0.002
RMSD bond angle	0.542
Data reduction software	HKL-2000 (v720)
Data scaling software	HKL-2000 (v720)
Phasing software	PHENIX (1.19.2_4158)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.090
High resolution limit [Å]	2.050	2.050
Number of reflections	11974	294
<I/σ(I)>	38.4	1.4
Completeness [%]	99.8	97.3
Redundancy	22	12.4
CC(1/2)	0.992	0.721

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.1 M MOPS, pH 7.0; 9% PEG 8000; 20 mM MgCl2, 5 mM ATP