7MSN
SunS glycosin S-glycosyltransferase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2011-04-10 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 0.9787 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 108.730, 108.730, 210.697 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.380 - 3.000 |
R-factor | 0.2079 |
Rwork | 0.204 |
R-free | 0.27960 |
Structure solution method | SAD |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.100 |
High resolution limit [Å] | 3.000 | 3.000 |
Number of reflections | 47618 | 2476 |
<I/σ(I)> | 20.1 | |
Completeness [%] | 91.2 | |
Redundancy | 7.4 | |
CC(1/2) | 0.998 | 0.800 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 283 | PEG 4000 50 mM HEPES, pH=7.5 |