7MSL
Structure of anti-CRISPR AcrIIC4 from Haemophilus parainfluenzae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | Cu FINE FOCUS |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-02-02 |
| Detector | RIGAKU |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 82.450, 82.450, 27.340 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.870 - 1.730 |
| R-factor | 0.2422 |
| Rwork | 0.239 |
| R-free | 0.27360 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.536 |
| Data reduction software | MOSFLM |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.870 | 1.800 |
| High resolution limit [Å] | 1.730 | 1.730 |
| Number of reflections | 10268 | 990 |
| <I/σ(I)> | 17.32 | |
| Completeness [%] | 99.9 | |
| Redundancy | 2 | |
| CC(1/2) | 0.998 | 0.670 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 294.15 | 35% PEG 600, 0.1M NaHPO4/citric acid pH 4.2 |
