7MSJ
The crystal structure of mouse HVEM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-11-19 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 64.656, 64.656, 69.043 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.100 |
| R-factor | 0.2142 |
| Rwork | 0.212 |
| R-free | 0.25750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4fhq |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.928 |
| Data reduction software | XDS |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0123) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 5.700 | 2.100 |
| Rmerge | 0.135 | 0.069 | 0.938 |
| Rmeas | 0.141 | 0.072 | 0.986 |
| Rpim | 0.038 | 0.021 | 0.295 |
| Total number of observations | 120946 | ||
| Number of reflections | 8989 | 500 | 422 |
| <I/σ(I)> | 11 | ||
| Completeness [%] | 99.5 | 94 | 99.1 |
| Redundancy | 13.5 | 11.6 | 9.9 |
| CC(1/2) | 0.997 | 0.943 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.5 | 291 | 0.2 M lithium sulfate, 0.1 M sodium acetate pH 4.5, 30% PEG 8000, 10% NDSB-211 |
