7MRQ
Chicken CNTN4 FN1-FN3 domains with T751A, V752A, Y781A, E786A mutations
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-08-21 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 71.010, 91.460, 124.290 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.770 - 3.200 |
R-factor | 0.225 |
Rwork | 0.221 |
R-free | 0.26100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5e4s |
RMSD bond length | 0.005 |
RMSD bond angle | 0.820 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.310 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.193 | 0.767 |
Rmeas | 0.202 | 0.812 |
Number of reflections | 13597 | 1014 |
<I/σ(I)> | 12 | 1.9 |
Completeness [%] | 95.1 | 71.7 |
Redundancy | 11.3 | |
CC(1/2) | 0.980 | 0.846 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 4.2M NaCl, 2 (v/v) % Glycerol |