7MQM
AAC(3)-IIIa in complex with CoA and gentamicin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | LIQUID ANODE |
| Source details | BRUKER METALJET |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-01-27 |
| Detector | Bruker PHOTON II |
| Wavelength(s) | 1.3418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 130.574, 91.059, 100.524 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.048 - 1.600 |
| R-factor | 0.1976 |
| Rwork | 0.197 |
| R-free | 0.21610 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.741 |
| Data reduction software | PROTEUM PLUS (2018.7-2) |
| Data scaling software | PROTEUM PLUS (2018.7-2) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | PHENIX ((1.15.1_3469: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.050 | 1.657 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 157949 | 15589 |
| <I/σ(I)> | 42.26 | 6.04 |
| Completeness [%] | 99.4 | 99.7 |
| Redundancy | 24 | |
| CC(1/2) | 0.997 | 0.207 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295.15 | 0.1M MES pH 6.5, 30% w/v PEG 4000, 3% v/v 1,3-propanediol |
