7MPZ

HNH Nuclease Domain from G. stearothermophilus Cas9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	RIGAKU MICROMAX-003
Temperature [K]	100
Detector technology	CCD
Collection date	2019-09-05
Detector	RIGAKU SATURN 944
Wavelength(s)	1.541780
Spacegroup name	P 41 21 2
Unit cell lengths	57.956, 57.956, 119.393
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.760 - 2.040
R-factor	0.1958
Rwork	0.194
R-free	0.23770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6j9n
RMSD bond length	0.006
RMSD bond angle	0.817
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17rc4_3626)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.760	2.110
High resolution limit [Å]	2.040	2.040
Number of reflections	24738	2458
<I/σ(I)>	20.31
Completeness [%]	99.8	99.39
Redundancy	7.3	5.6
CC(1/2)	1.000	0.539

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.12 M [0.3 M diethylene glycol, 0.3 M triethylene glycol, 0.3 M tetraethylene glycol, 0.3 M pentaethylene glycol], 0.1 M [MES and imidazole] pH 6.5, 30% (v/v) [40% (v/v) glycerol, 20% (w/v) polyethylene glycol 4000]