7MPX
CmcC E36G mutant from Type II Cut MCP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-08-18 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9786 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 66.570, 61.000, 66.610 |
Unit cell angles | 90.00, 119.92, 90.00 |
Refinement procedure
Resolution | 57.730 - 2.100 |
R-factor | 0.2419 |
Rwork | 0.237 |
R-free | 0.28320 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | D_1000255588 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (2.8.3) |
Refinement software | PHENIX (1.18.2) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 57.730 | 57.730 | 2.150 |
High resolution limit [Å] | 2.100 | 9.370 | 2.100 |
Rmerge | 0.105 | 0.042 | 1.430 |
Rmeas | 0.118 | 0.047 | 1.597 |
Total number of observations | 134627 | ||
Number of reflections | 26358 | 325 | 1920 |
<I/σ(I)> | 6.83 | 23.29 | 0.87 |
Completeness [%] | 96.1 | 97 | 95.3 |
Redundancy | 5.108 | 4.822 | 4.968 |
CC(1/2) | 0.998 | 0.999 | 0.730 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 294 | 0.1 M SPG buffer pH 6.0, 25% PEG 1500 |