7MPR
Brucella melitensis NrnC
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-03-26 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 4 2 2 |
Unit cell lengths | 93.320, 93.320, 123.973 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 74.560 - 1.420 |
Rwork | 0.165 |
R-free | 0.18100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1yt3 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.718 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19_4080) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 74.560 | 1.471 |
High resolution limit [Å] | 1.420 | 1.420 |
Number of reflections | 103308 | 10058 |
<I/σ(I)> | 30.58 | 1.91 |
Completeness [%] | 99.8 | 98.78 |
Redundancy | 15.3 | 10.9 |
CC(1/2) | 1.000 | 0.782 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 294 | 0.1 M HEPES, 2.0 M Ammonium Sulfate, 19 % xylitol |