7MPN
Bartonella henselae NrnC bound to pGC
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-10-19 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0001 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 100.078, 142.387, 148.839 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.210 - 1.940 |
R-factor | 0.1946 |
Rwork | 0.194 |
R-free | 0.22500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1yt3 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.767 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.15.2_3471) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.210 | 2.015 |
High resolution limit [Å] | 1.940 | 1.945 |
Number of reflections | 155943 | 15333 |
<I/σ(I)> | 9.08 | |
Completeness [%] | 99.7 | 99.13 |
Redundancy | 6.6 | |
CC(1/2) | 0.998 | 0.778 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 294 | 0.1 M Succinic acid; 10% PEG 3,350; 20% xylitol |