7MP9
Crystal structure of the cytosolic domain of Tribolium castaneum PINK1 phosphorylated at Ser205 in complex with ADP analog
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-03-14 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97949 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 52.736, 52.736, 547.944 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.670 - 2.800 |
R-factor | 0.2497 |
Rwork | 0.247 |
R-free | 0.30650 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5yj9 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.548 |
Data reduction software | autoPROC |
Data scaling software | STARANISO |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.670 | 2.920 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 9568 | 478 |
<I/σ(I)> | 10.6 | 2.3 |
Completeness [%] | 93.4 | 100 |
Redundancy | 34.9 | |
CC(1/2) | 0.996 | 0.841 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 288 | 80mM MES (pH 6.5), 0.16M (NH4)2 SO4, 16% PEG 8K. |