7MO5

Crystal Structure of the ZnF4 of Nucleoporin NUP153 in complex with Ran-GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-29
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.03317
Spacegroup name	P 21 21 2
Unit cell lengths	59.780, 80.110, 54.480
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.000 - 1.550
R-factor	0.1634
Rwork	0.162
R-free	0.19010
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	xia2
Phasing software	AutoSol
Refinement software	PHENIX (1.18.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.000	28.010	1.610
High resolution limit [Å]	1.550	3.340	1.550
Rmeas	0.075	0.042	1.871
Rpim	0.021	0.012	0.510
Total number of observations		48567	50078
Number of reflections	73272	4095	3823
<I/σ(I)>	17.7	50.6	1.5
Completeness [%]	99.9	100	99.8
Redundancy	12.9	11.9	13.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2	294	22% w/v PEG3350, 0.1 M Bis-Tris