7MO5
Crystal Structure of the ZnF4 of Nucleoporin NUP153 in complex with Ran-GDP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-10-29 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.03317 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 59.780, 80.110, 54.480 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.000 - 1.550 |
R-factor | 0.1634 |
Rwork | 0.162 |
R-free | 0.19010 |
Structure solution method | SAD |
Data reduction software | XDS |
Data scaling software | xia2 |
Phasing software | AutoSol |
Refinement software | PHENIX (1.18.2) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 28.000 | 28.010 | 1.610 |
High resolution limit [Å] | 1.550 | 3.340 | 1.550 |
Rmeas | 0.075 | 0.042 | 1.871 |
Rpim | 0.021 | 0.012 | 0.510 |
Total number of observations | 48567 | 50078 | |
Number of reflections | 73272 | 4095 | 3823 |
<I/σ(I)> | 17.7 | 50.6 | 1.5 |
Completeness [%] | 99.9 | 100 | 99.8 |
Redundancy | 12.9 | 11.9 | 13.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 294 | 22% w/v PEG3350, 0.1 M Bis-Tris |