7MO4
Crystal Structure of the ZnF3 of Nucleoporin NUP153 in complex with Ran-GDP, resolution 2.4 Angstrom
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-12-11 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.660, 61.240, 69.750 |
| Unit cell angles | 90.00, 104.04, 90.00 |
Refinement procedure
| Resolution | 29.440 - 2.400 |
| R-factor | 0.2154 |
| Rwork | 0.214 |
| R-free | 0.23680 |
| Structure solution method | SAD |
| Data reduction software | XDS |
| Data scaling software | xia2 |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.18.2) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 29.440 | 29.440 | 2.490 |
| High resolution limit [Å] | 2.400 | 5.160 | 2.400 |
| Rmeas | 0.086 | 0.059 | 1.463 |
| Rpim | 0.033 | 0.023 | 0.552 |
| Total number of observations | 14032 | 14427 | |
| Number of reflections | 41648 | 2201 | 2104 |
| <I/σ(I)> | 11.3 | 26.5 | 1.3 |
| Completeness [%] | 98.6 | 97.9 | 98.5 |
| Redundancy | 6.7 | 6.4 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.9 | 294 | 20% w/v PEG3350, 0.1 M Bis-Tris |
