7MO4

Crystal Structure of the ZnF3 of Nucleoporin NUP153 in complex with Ran-GDP, resolution 2.4 Angstrom

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-11
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 1 21 1
Unit cell lengths	67.660, 61.240, 69.750
Unit cell angles	90.00, 104.04, 90.00

Refinement procedure

Resolution	29.440 - 2.400
R-factor	0.2154
Rwork	0.214
R-free	0.23680
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	xia2
Phasing software	AutoSol
Refinement software	PHENIX (1.18.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.440	29.440	2.490
High resolution limit [Å]	2.400	5.160	2.400
Rmeas	0.086	0.059	1.463
Rpim	0.033	0.023	0.552
Total number of observations		14032	14427
Number of reflections	41648	2201	2104
<I/σ(I)>	11.3	26.5	1.3
Completeness [%]	98.6	97.9	98.5
Redundancy	6.7	6.4	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.9	294	20% w/v PEG3350, 0.1 M Bis-Tris