7MNS

Crystal Structure of the ZnF4 of Nucleoporin NUP358/RanBP2 in complex with Ran-GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-04-13
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.28210
Spacegroup name	P 65 2 2
Unit cell lengths	92.480, 92.480, 194.780
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.910 - 2.100
R-factor	0.1761
Rwork	0.175
R-free	0.20250
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	xia2
Phasing software	AutoSol
Refinement software	PHENIX (1.18.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.910	29.920	2.180
High resolution limit [Å]	2.100	4.520	2.100
Rmeas	0.181	0.052	2.315
Rpim	0.035	0.010	0.448
Total number of observations	754508	75845	73905
Number of reflections	29596	3246	2860
<I/σ(I)>	21.2	64.4	1.8
Completeness [%]	100.0	100	99.9
Redundancy	25.5	23.4	25.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	294	18 % (w/v) PEG 3,350; 0.1M Bis-Tris