7MNQ

Crystal Structure of the ZnF2 of Nucleoporin NUP358/RanBP2 in complex with Ran-GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-09
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.2829
Spacegroup name	P 21 21 2
Unit cell lengths	59.820, 78.660, 56.270
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.130 - 2.050
R-factor	0.1906
Rwork	0.190
R-free	0.20850
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	xia2
Phasing software	AutoSol
Refinement software	PHENIX (1.18.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.140	28.140	2.120
High resolution limit [Å]	2.050	4.410	2.050
Rmeas	0.104	0.058	2.088
Rpim	0.029	0.016	0.607
Total number of observations	215148	22091	17443
Number of reflections	17126	1854	1596
<I/σ(I)>	13.9	36.4	1.2
Completeness [%]	99.4	100	95.6
Redundancy	12.6	11.9	10.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	294	21% (w/v) PEG 3350; 0.1 M Bis-Tris