7MNI

Crystal structure of the N-terminal domain of NUP88 in complex with NUP98 C-terminal Autoproteolytic Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-08-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.000030
Spacegroup name	P 1
Unit cell lengths	59.748, 72.085, 73.042
Unit cell angles	92.53, 103.69, 100.39

Refinement procedure

Resolution	20.000 - 2.000
R-factor	0.1767
Rwork	0.175
R-free	0.20840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB entries 5CWW & 1KO6
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	PHENIX (1.17.1)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000	20.000	2.040
High resolution limit [Å]	2.000	10.000	2.000
Rmerge	0.131	0.078	0.984
Rmeas	0.143	0.084	1.157
Rpim	0.056	0.031	0.604
Total number of observations	456229	3776	16182
Number of reflections	76633	541	4472
<I/σ(I)>	7.5	23	1.3
Completeness [%]	97.8	86.4	96.6
Redundancy	6	7	3.6
CC(1/2)	0.996	0.995	0.513

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.3	294	20% w/v PEG4000, 0.22 M sodium chloride, 0.1 M Tris